Synergene

 

Standard Primer List

Primer Name Nucleotide Sequence Comments/Usage
T3 AATTAACCCTCACTAAAGGG Universal Primer
T7 TAATACGACTCACTATAGGG Universal Primer
T7term GCTAGTTATTGCTCAGCGGT Universal Primer
SP6 ATTTAGGTGACACTATAG Universal Primer
M13(-20)f GTAAAACGACGGCCAGT Universal Primer
M13rev GGAAACAGCTATGACCATG Universal Primer
pUC18rev GGATAACAATTTCACACAGG Universal Primer
SV40rev GTCACACCACAGAAGTAAGG Universal, also known as pUASTr
hsp70(+60)f CGCAGCTGAACAAGCTAAACAATC Universal, also known as pUASTf
hsp70(+60)r GATTGTTTAGCTTGTTCAGCTGCG Universal Primer
P3 ACTATTCCTTTCACTCGCAC P-element primer(3'Side)
pQE-fwd CCCGAAAAGTGCCACCTG fwd Primer for Qiagen pQE Vectors
pQE-rev GTTCTGAGGTCATTACTGG rev Primer for Qiagen pQE Vectors
pQE-mcs CGGATAACAATTTCACACAG fwd Primer for Qiagen pQE Vectors very close to MCS
A GAGGCCCTTTCGTCTTCAAG P-element primer
B CAATAGTACAGAGAGGGAGAG Sequencing P{GawB} plasmid rescues
C CTATGGAAAAACGCCAGCAACG Sequencing P{GawB} plasmid rescues
D TTCGGCTATCGACGGGACCAC Sequencing plasmid rescues
E TCAGGGGGGCGGAGCCTATG P-element primer
P CGACGGGACCACCTTATGTTATTTCATCATG inv-PCR from P-elements
Pshort CGACGGGACCACCTTATG Sequencing Plasmid rescues
Prev CATGATGAAATAACATAAGGTGGTCCCGTCG Reverse version of P
MJE4 GAGTCTGAGTGAGACAGCG inv-PCR from P-elements
tub1 CACTGATTTCGACGGTTACCC sequencing inserts in front of tub-trailer
pGEX-3 CCGGGAGCTGCATGTGTCAGAGG Sequencing C-terminus of fusion protein
pGEX-5 GGGCTGGCAAGCCACGTTTGGTG Sequencing GST-Gene Fusion site
flu-1 CTCGGTCGACGCGCGCTACCCATACGATGTTCCTG HA-tagged constructs
EP02 CTTACTGAACGGTGATCCC Sequencing EP-line Plasmid rescues
pcDNA3.1rev TAGAAGGCACAGTCGAGG Reverse primer for pcDNA3.1/BGH Invitrogen vectors